Complete sequence of the bithorax complex of Drosophila

Creators: Martin, Christopher H.; Mayeda, Carol A.; Davis, Cheryl A.; Ericsson, Cheryl L.; Knafels, John D.; Mathog, David R.; Celniker, Susan E.; Lewis, Edward B.; Palazzolo, Michael J.

Abstract

The bithorax complex (BX-C) of Drosophila, one of two complexes that act as master regulators of the body plan of the fly, is included within a sequence of 338,234 bp (SEQ89E). This paper presents the strategy used in sequencing SEQ89E and an analysis of its open reading frames. The BX-C sequence (BXCALL) contains 314,895 bp obtained by deletion of putative genes that are located at each end of SEQ89E and appear to be functionally unrelated to the BX-C. Only 1.4% of BXCALL codes for the three homeodomain-containing proteins of the complex. Principal findings include a putative ABD-A protein (ABD-AII) larger than a previously known ABD-A protein and a putative glucose transporter-like gene (1521 bp) located at or near the bithoraxoid (bxd), infra-abdominal-2 (iab-2) boundary on the opposite strand relative to that of the homeobox-containing genes.

Additional Information

Copyright © 1995 by the National Academy of Sciences. Contributed by Edward B. Lewis, June 19, 1995. We thank the sequencing group of the Berkeley Drosophila Genome Project, including Michelle Lee, Vivi Truong, Rebecca Zhang, Melvin Li, Kathryn Gunning, Annie Chiang, Marnel Bondoc, Kala Kowtha, Migdad Machrus, and Tom Cloutier, for their superb technical assistance. We thank Joe Jaklevic and his Lawrence Berkeley Laboratory Human Genome Center Automation group; Frank Eeckman, Ed Theil, and the Lawrence Berkeley Laboratory Human Genome Center Informatics group; Bill Kimmerly and the Drosophila Physical Mapping Group; and Suzanna Lewis and the Drosophila Genome Center Informatics group for their contributions to the sequencing effort. Analysis of the sequence was done by the California Institute of Technology group. We thank Mary Raney, Victor Hsu, Gretl Hornung, Mallory Zhang, and John Hubenschmidt for technical assistance. We thank Welcome Bender, Mike Kuziora, Michele Lamka, Kim McCall, and Shige Sakonju for communicating their unpublished results. We thank Welcome Bender, Howard Lipshitz, and Joanne Topol for critical reading of the manuscript. The sequencing was done by the Berkeley Drosophila Genome Project, which is a consortium of the Drosophila Genome Center [supported by grants from the National Center for Human Genome Research (P50-HG00750 to Gerald Rubin and HG00837-03 to M.J.P. and C.H.M.)] and the Human Genome Center of the Lawrence Berkeley Laboratory, Mohan Narla, Acting Director (supported by the U.S. Department of Energy). M.J.P. is a Lucille P. Markey Scholar and his effort was funded in part by the Lucille P. Markey Charitable Trust. The analysis was supported by research grants to E.B.L. from the National Institutes of Health (HD06331 and HD30727) and the March of Dimes. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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