High-speed widefield photoacoustic microscopy of small-animal hemodynamics
Abstract
Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest.
Additional Information
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 25 Jul 2018; revised 3 Sep 2018; accepted 4 Sep 2018; published 7 Sep 2018. Funding: NIH (NS099590 and NS097554); AHA (18CSA34080277); Duke University MEDx fund. We would like to acknowledge the support of NIH grants NS099590 and NS097554 (to W.Y.), AHA collaborative sciences grant 18CSA34080277 (to W.Y., J.Y., and U.H.), and Duke University MEDx fund (to J.Y.). The authors declare that there are no conflicts of interest related to this article.Attached Files
Published - boe-9-10-4689.pdf
Supplemental Material - Video_1-Polygonscan.avi
Supplemental Material - Video_2-Ear-Epinephrine-Polygon-v1_0.avi
Supplemental Material - Video_3-Brain-stroke-Polygon-v3_0.avi
Files
Additional details
- PMCID
- PMC6179413
- Eprint ID
- 90275
- Resolver ID
- CaltechAUTHORS:20181016-091038256
- NS099590
- NIH
- NS097554
- NIH
- 18CSA34080277
- American Heart Association
- Duke University
- Created
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2018-10-16Created from EPrint's datestamp field
- Updated
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2022-03-07Created from EPrint's last_modified field