A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila

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Acknowledgement

We thank the Bloomington Drosophila Stock Center and the Developmental Studies Hybridoma Bank for reagents. We thank Z. Zhang and N. Lau for their helpful discussions and the Yamashita lab members for comments on the paper. The research was supported by the Howard Hughes Medical Institute (Y.M.Y., P.D.Z. and S.E.J.), National Institute of Health (NIH R01 HD109667 to J.K.K., R35 GM136275 to P.D.Z., R01GM097363 to A.A.A. and R35 GM130272 to S.E.J.) and the Whitehead Institute for Biomedical Research (Y.M.Y.).

Contributions

These authors contributed equally: Zsolt G. Venkei, Ildar Gainetdinov.

Z.G.V. and Y.M.Y. conceived the project. Z.G.V., I.G., S.E.J., J.K.K., P.D.Z. and Y.M.Y. designed experiments and interpreted the results. Z.G.V., I.G., A.B., C.B., J.M.F. and Y.M.Y. conducted experiments. Z.G.V., I.G., M.R.S., C.P.C., T.W.W., G.W.B. and S.F. analysed data. P.C. and A.A.A. contributed critical information in the course of the investigation. Z.G.V., I.G., Y.M.Y. and P.D.Z. wrote and edited the paper with the input from other authors. Y.M.Y. and P.D.Z. supervised the research.

Data Availability

Sequencing data generated in this study have been deposited in the National Center for Biotechnology Information Short Read Archive database under accession code PRJNA879723. Fly genome sequence and annotation (build dm6/BDGP6.22 release 98) used in this study were downloaded from Ensembl at ftp://ftp.ensembl.org/pub/release-98/fasta/drosophila_melanogaster/ and ftp://ftp.ensembl.org/pub/release-98/gtf/drosophila_melanogaster/; fly rRNA sequences were downloaded from SILVA rRNA database at https://www.arb-silva.de/. Source data are provided with this paper. All other data supporting the findings of this study are available from the corresponding authors upon request.

Code Availability

Code used in this work is deposited at https://github.com/ildargv/Venkei_et_al_2023.