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Published October 15, 2011 | Published
Journal Article Open

Microscopy refocusing and dark-field imaging by using a simple LED array

Abstract

The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

Additional Information

© 2011 Optical Society of America. Received July 14, 2011; revised September 9, 2011; accepted September 12, 2011; posted September 13, 2011 (Doc. ID 151079); published October 5, 2011. We acknowledge funding support from United States Department of Defense under grant W81XWH-09-1-0051.

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