Diffraction tomography with Fourier ptychography
Abstract
This paper presents a technique to image the complex index of refraction of a sample across three dimensions. The only required hardware is a standard microscope and an array of LEDs. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. The experimental microscope demonstrates a lateral spatial resolution of 0.39 μm and an axial resolution of 3.7 μm at the Nyquist–Shannon sampling limit (0.54 and 5.0 μm at the Sparrow limit, respectively) across a total imaging depth of 110 μm. Unlike competing methods, this technique quantitatively measures the volumetric refractive index of primarily transparent and contiguous sample features without the need for interferometry or any moving parts. Wide field-of-view reconstructions of thick biological specimens suggest potential applications in pathology and developmental biology.
Additional Information
© 2016 Optical Society of America. Received 2 May 2016; revised 3 July 2016; accepted 3 July 2016 (Doc. ID 264094); published 27 July 2016. The authors would like to thank J. Brake, B. Judkewitz, and I. Papadopoulos for the helpful discussions and feedback. Funding: National Institutes of Health (NIH) (1R01AI096226-01); The Caltech Innovation Initiative (CI2) Program (13520135).Attached Files
Accepted Version - nihms835228.pdf
Supplemental Material - 827__1_.MP4
Supplemental Material - 827.MP4
Supplemental Material - optica-3-8-827.pdf
Files
Additional details
- PMCID
- PMC5521281
- Eprint ID
- 70694
- Resolver ID
- CaltechAUTHORS:20160930-124858247
- NIH
- 1R01AI096226-01
- Caltech Innovation Initiative (CI2)
- 13520135
- Created
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2016-09-30Created from EPrint's datestamp field
- Updated
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2022-04-26Created from EPrint's last_modified field