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Published October 2010 | Published + Accepted Version
Journal Article Open

Integrated Photoacoustic and Fluorescence Confocal Microscopy


We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.

Additional Information

© 2010 IEEE. Manuscript received May 1, 2010; revised June 11, 2010; accepted June 17, 2010. Date of publication July 15, 2010; date of current version September 15, 2010. This work was supported in part by the National Institutes of Health under Grant R01 EB000712, Grant R01 EB008085, Grant R01 CA113453901, Grant U54 CA136398, and Grant 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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Published - 05510116.pdf

Accepted Version - nihms243293.pdf


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August 19, 2023
October 20, 2023