Picosecond absorption relaxation measured with nanosecond laser photoacoustics
Picosecond absorption relaxation—central to many disciplines—is typically measured by ultrafast (femtosecond or picosecond) pump-probe techniques, which however are restricted to optically thin and weakly scattering materials or require artificial sample preparation. Here, we developed a reflection-mode relaxation photoacoustic microscope based on a nanosecond laser and measured picosecond absorptionrelaxation times. The relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, were measured at 576 nm. The added advantages in dispersion susceptibility, laser-wavelength availability, reflection sensing, and expense foster the study of natural—including strongly scattering and nonfluorescent—materials.
Additional Information© 2010 American Institute of Physics. Received 21 July 2010; accepted 16 September 2010; published online 18 October 2010. This work was sponsored in part by National Institutes of Health under Grant Nos. R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Published - 1.3500820.pdf