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Published October 31, 2016 | Published
Journal Article Open

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Abstract

Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample's Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase image is obtained after a deconvolution process with a minimum of two phase gradient images. Here, we introduce PMDPC quantitative phase image reconstruction algorithm and realize it experimentally in a 4f system with an SLM placed at the pupil plane. In our current experimental setup with the numerical aperture of 0.36, we obtain a quantitative phase image with a resolution of 1.73μm after computationally removing system aberrations and refocusing. We also extend the depth of field digitally by 20 times to ±50μm with a resolution of 1.76μm.

Additional Information

© 2016 Optical Society of America. Received 26 Jul 2016; revised 15 Oct 2016; accepted 18 Oct 2016; published 21 Oct 2016. The authors would like to thank Daniel Martin for fabricating the Siemens star phase target, and thanks to Jinho Kim, Joshua Brake and Haowen Ruan for discussions and help with experiments. Funding: NIH (grant no. R01 AI096226); CII 2015 (grant no. 32070065).

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