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Published January 15, 1990 | Published
Journal Article Open

Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein


Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA polymerase II. Single-stranded DNA-binding activity is associated with the 66-kDa polypeptide.

Additional Information

© 1990 National Academy of Sciences. Communicated by Norman Davidson, October 24, 1989 (received for review August 30, 1989). This work was supported by grants from the National Institutes of Health (GM25508) and the American Cancer Society (NP-623G). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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