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Published October 20, 2014 | Published + Accepted Version
Journal Article Open

Multiview optical resolution photoacoustic microscopy


Optical resolution photoacoustic microscopy (OR-PAM), while providing high lateral resolution, has been limited by its relatively poor acoustically determined axial resolution. Although this limitation has been tackled in recent works by using either broadband acoustic detection or nonlinear photoacoustic effects, a flexible solution with three-dimensional optical resolution in reflection mode remains desired. Herein we present a multiview OR-PAM technique. By imaging the sample from multiple view angles and reconstructing the data using a multiview deconvolution method, we have experimentally demonstrated an isotropic optical resolution in three dimensions.

Additional Information

© 2014 Optical Society of America. Received 27 June 2014; revised 26 August 2014; accepted 26 August 2014 (Doc. ID 214941); published 29 September 2014. Funding: National Institutes of Health (NIH) (DP1 EB016986, R01 CA159959, R01 CA186567, R01 EB016963). The authors thank Prof. James Ballard for his close reading of the manuscript and an author of Ref. [15], Dr. Stephan Preibisch, for discussion of multiview deconvolution algorithms. We also thank Sarah DeGenova and Kelly Monk for helping prepare the zebrafish embryos. L. V. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

Attached Files

Published - optica-1-4-217.pdf

Accepted Version - nihms633992.pdf


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August 20, 2023
October 20, 2023