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Published July 1, 1957 | public
Journal Article Open

Equilibrium sedimentation of macromolecules in density gradients


This communication presents a new method for the study of the molecular weight and partial specific volume of macromolecules, with some illustrations based on results with deoxyribonucleic acid (DNA) and several viruses. The method involves observation of the equilibrium distribution of macromolecular material in a density gradient itself at equilibrium. The density gradient is established by the sedimentation of a low-molecular-weight solute in a solution subject to a constant centrifugal field.

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© 1957 by the National Academy of Sciences. Communicated by Linus Pauling, May 27, 1957. We gratefully acknowledge the technical assistance of Mr. Richard Holmquist, Mrs. Ilga Liclausis, and Mrs. Janet Morris. Mr. Darwin Smith rendered valuable assistance with the photometry. We are indebted to Dr. John Lamperti and to Mr. Charles Steinberg for many valuable discussions. Aided by grants from the National Institutes of Health and the National Foundation for Infantile Paralysis. [M.M. was a] Predoctoral Fellow of the National Science Foundation. [F.W.S. was a]Fellow in the Medical Sciences of the National Research Council. Gates and Crellin Laboratories of Chemistry, Contribution No. 2205.


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