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Published April 1, 2011 | Published + Accepted Version
Journal Article Open

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure


We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO_2) and oxygen partial pressure (pO_2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO_2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO_2 using phosphorescence quenching. The variations of sO_2 and pO_2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen–hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

Additional Information

© 2011 Optical Society of America. Received December 15, 2010; revised January 29, 2011; accepted February 18, 2011; posted February 23, 2011 (Doc. ID 139708); published March 16, 2011. We thank C. Zhang for helping with data processing and manuscript preparation. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. L. Wang has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

Attached Files

Published - ol-36-7-1029.pdf

Accepted Version - nihms281744.pdf


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