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Published December 2008 | public
Journal Article

Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices


Herein we report on reliable reproducible quantification of protein analytes in human serum by fluorescence sandwich immunoassays in disposable PDMS microfluidic chips. The system requires 1000 times less sample than typical clinical blood tests and is specifically shown to measure ferritin down to 250 pM in human serum. The in-built calibration method of spiking the serum with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The reported microfluidic system is an important new tool for fundamental scientific research, offering sensitive immunoassay measurements in small but complex biosamples. The system is also a further step towards comprehensive affordable "point-of-care" biomedical diagnostics.

Additional Information

© 2008 WILEY-VCH Verlag GmbH & Co. Received May 13, 2008, Revised June 19, 2008, Accepted June 23, 2008. The authors thank Christina Morales and Anna Chetverikova of the Caltech Microfluidic Foundry for their help with device fabrication. The authors also thank Avril Cunningham of the USC Leavy Library for her help with the reference material. Financial support for this work was provided by NIH Grants 1K99EB007151 and 1RO1HG002644 and the Caltech SURF Program. The authors have declared no conflict of interest.

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