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Published May 1978 | Published
Journal Article Open

Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA


Fragments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.

Additional Information

© 1978 National Academy of Sciences. Communicated by Eugene P. Kennedy, March 7, 1978. We thank O. Ritter for able technical assistance. The portion of this work carried out at Harvard Medical School was supported by Grant NP-1G from the American Cancer Society. The portion of this work carried out at Brookhaven National Laboratory was under the auspices of the Department of Energy and was supported by National Institutes of Health Research Grant GM-21872.

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Published - PNAS-1978-Campbell-2276-80.pdf


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