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Published July 1, 2010 | Published
Journal Article Open

Wide field-of-view microscope based on holographic focus grid illumination

Abstract

We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6mm×5mm and acquisition time of 2.5s. The resolution is fundamentally limited by the spot size—our demonstrated average FWHM spot diameter was 0.74μm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems

Additional Information

© 2010 Optical Society of America. Received March 22, 2010; revised May 19, 2010; accepted May 29, 2010; posted June 7, 2010 (Doc. ID 125762); published June 23, 2010. This work is supported by Department of Defense grant W81XWH-09-1-0051. The authors acknowledge Richard Cote and Ram Datar from the University of Miami for helpful discussions.

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