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Published February 17, 2020 | Published
Book Section - Chapter Open

In vivo super-resolution photoacoustic computed tomography by localization of single dyed droplets


The spatial resolution of photoacoustic (PA) computed tomography (PACT) is limited by acoustic diffraction. Here, we report in vivo superresolution PACT, which breaks the acoustic diffraction limit by localizing the centers of single dyed droplets. The dyed droplets generate much stronger PA signals than blood and can flow smoothly in blood vessels; thus, they are excellent tracers for localization-based superresolution imaging. The flowing droplets were first localized, and then their center positions were used to construct a superresolution image that exhibits sharper features and more finely resolved vascular details. A 6-fold improvement in spatial resolution has been realized in vivo.

Additional Information

© 2020 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by the NIH grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), U01 NS090579 (BRAIN Initiative), and U01 NS099717 (BRAIN Initiative). L.W. has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. The other authors declare no conflict of interest.

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