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Published November 1978 | Published
Journal Article Open

Mutants of Sindbis virus. III. Host polypeptides present in purified HR and ts103 virus particles


The amounts of host-encoded protein present in purified Sindbis virions of both the HR strain and of a mutant (ts103) which makes multicored particles were examined. Cells were labeled with [^(35)S]methionine before infection and with [^(3)H]methionine postinfection. Virions were purified by velocity sedimentation and isopycnic banding, and their polypeptides were examined by polyacrylamide gels in a sodium dodecyl sulfate-containing discontinuous buffer system. Host prelabeled material was found principally in a small number of discrete polypeptides in HR virions, which contained as little as 0.2% host-encoded protein. Virus-sized particles of mutant ts103 contained significantly more host material, and multiploid particles from ts103 infection contained up to 12% host prelabeled protein.

Additional Information

© 1978 American Society for Microbiology. Received for publication 5 June 1978. I thank Edith Lenches and Mary Stammreich-Martin for excellent technical assistance, Charles Rice for his participation in the preliminary experiments, and James H. Strauss for stimulating discussions and invaluable assistance in the preparation of this manuscript. This research was supported by Public Health Service grant AI-10793 from the National Institute of Allergy and Infectious Diseases.

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