Published November 2, 2009
| Accepted Version + Supplemental Material
Journal Article
Open
Generation of Surface-Bound Multicomponent Protein Gradients
- Creators
- Zhang, Kechun
- Sugawara, Ayae
-
Tirrell, David A.
Abstract
Spatial control of bioactive ligands is achieved by integrating microfluidics and protein engineering. The proteins of interest are mixed in a gradient generator and immobilized on artificial polypeptide scaffolds through the strong association of heterodimeric ZE/ZR leucine zipper pairs. Protein densities and gradient shapes are easily controlled and varied in this method.
Additional Information
© 2009 WILEY. Received: 28 August 2009; published Online: 24 Sep 2009. This work was supported by the NSF-sponsored Center for the Science and Engineering of Materials at the California Institute of Technology, and by NIH grant GM62523.Attached Files
Accepted Version - nihms415121.pdf
Supplemental Material - sm001.pdf
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Additional details
- PMCID
- PMC3517104
- Eprint ID
- 16886
- DOI
- 10.1002/cbic.200900542
- Resolver ID
- CaltechAUTHORS:20091207-100800458
- NSF
- GM62523
- NIH
- Created
-
2009-12-08Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field