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Published September 1, 1990 | Published
Journal Article Open

Alternative splicing produces transcripts encoding two forms of the α subunit of GTP-binding protein G_o


The α subunit of the guanine nucleotide-binding protein G_o ("o" for other) is believed to mediate signal transduction between a variety of receptors and effectors. cDNA clones encoding two forms of G_o α subunit were isolated from a mouse brain library. These two forms, which we call G_(o)Aα and G_(o)Bα, appear to be the products of alternative splicing. G_(o)Aα differs from G_(o)Bα over the C-terminal third of the deduced protein sequence. Both forms are predicted to be substrates for ADP-ribosylation by pertussis toxin. G_(o)Aα transcripts are present in a variety of tissues but are most abundant in brain. The G_(o)Bα transcript is expressed at highest levels in brain and testis. It is possible that G_(o)Aα and G_(o)Bα have different functions.

Additional Information

© 1990 National Academy of Sciences. Contributed by Melvin I. Simon, June 4, 1990. We thank Carol Lee for technical assistance, Ryn Miake-Lye for RNA, Narasimhan Gautam for discussions, and T. H. Chang for critical reading of the manuscript. We are grateful to Y. Kaziro for communicating results before publication. This work was supported by National Institutes of Health Grant GM 34236.

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Published - PNAS-1990-Strathmann-6477-81.pdf


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August 19, 2023
October 19, 2023