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Published March 4, 2013 | Published
Book Section - Chapter Open

High resolution functional photoacoustic computed tomography of the mouse brain during electrical stimulation


Photoacoustic computed tomography (PACT) is an emerging imaging technique which is based on the acoustic detection of optical absorption from tissue chromophores, such as oxy-hemoglobin and deoxy-hemoglobin. An important application of PACT is functional brain imaging of small animals. The conversion of light to acoustic waves allows PACT to provide high resolution images of cortical vasculatures through the intact scalp. Here, PACT was utilized to study the activated areas of the mouse brain during forepaw and hindpaw stimulations. Temporal PACT images were acquired enabling computation of hemodynamic changes during stimulation. The stimulations were performed by trains of pulses at different stimulation currents (between 0.1 to 2 mA) and pulse repetition rates (between 0.05 Hz to 0.01Hz). The response at somatosensory cortex-forelimb, and somatosensory cortex-hindlimb, were investigated. The Paxinos mouse brain atlas was used to confirm the activated regions. The study shows that PACT is a promising new technology that can be used to study brain functionality with high spatial resolution.

Additional Information

© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors acknowledge and thank Prof. James Ballard, for his close look at the manuscript. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, R01 CA159959, U54 CA136398, R01 EB010049, R01 CA157277, and DP1 EB016986 (NIH Director's Pioneer Award). L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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