CaltechAUTHORS
Published November 12, 2007 | Version public
Journal Article Metadata-only

Engineered Alkane‐Hydroxylating Cytochrome P450_(BM3) Exhibiting Nativelike Catalytic Properties

Creators

Abstract

Divide, evolve, and conquer: A domain‐based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole‐cell bioconversions of propane to propanol under mild conditions, using air as oxidant.

Additional Information

© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: June 15, 2007; Published online: September 20, 2007. This work was supported by a Swiss National Science Foundation fellowship to R.F., a U.S. NSF Fellowship to M.M.C. and by the U.S. Army Research Office, ARO Contract DAAD19‐03‐D‐0004. We thank Dr. Christopher Snow for providing the homology model of P450_(BM3) reductase and Dr. Matthew W. Peters, Dr. Peter Meihnold, and Dr. Marco Landwehr for helpful discussions regarding the biotransformations and for providing access to DasGip fermenter.

Additional details

Additional titles

Alternative title
Engineered Alkane‐Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties

Identifiers

Eprint ID
89917
DOI
10.1002/anie.200702616
Resolver ID
CaltechAUTHORS:20180925-122611378

Related works

Describes
10.1002/anie.200702616 (DOI)

Funding

Swiss National Science Foundation (SNSF)
NSF
Army Research Office (ARO)
DAAD19‐03‐D‐0004

Dates

Created
2018-09-25
Created from EPrint's datestamp field
Updated
2021-11-16
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