Design and Evaluation of a Peptidyl Fluorescent Chemosensor for Divalent Zinc
- Creators
- Walkup, Grant K.
- Imperiali, Barbara
Abstract
The rapid analysis of trace metal cations for environmental and biomedical applications is particularly demanding since it requires the specific recognition of a particular element in the presence of numerous closely related species. While remarkable progress has been achieved for inorganic analytes, such as Ca^(2+), there is still a significant need for the genesis of new fluorosensors. In light of the selectivity and avidity with which proteins bind divalent metal cations, we have chosen to use a polypeptide architecture as the framework for metal ion recognition.In addition, our ability to manipulate synthetic polypeptides allows the coordinated integration of fluorescent reporters for signal transduction within a metal-binding peptidyl construct. Herein we report the design, synthesis, and evaluation of a selective fluorescent chemosensor, sensitive to nanomolar concentrations of Zn^(2+).
Additional Information
© 1996 American Chemical Society. Received November 16, 1995. Publication Date (Web): March 27, 1996. This research was supported by ONR, NSF, and the Caltech President's Fund. The award of a Glaxo Summer Fellowship (to G.K.W.) and a Camille and Henry Dreyfus Teacher Scholar Award (to B.I.) is also gratefully acknowledged.Attached Files
Supplemental Material - ja3053.pdf
Files
Name | Size | Download all |
---|---|---|
md5:9afe1ed7bab70616d3ac864ee30c8b44
|
482.2 kB | Preview Download |
Additional details
- Eprint ID
- 86417
- Resolver ID
- CaltechAUTHORS:20180515-155558284
- Office of Naval Research (ONR)
- NSF
- Caltech President's Fund
- Glaxo Summer Fellowship
- Camille and Henry Dreyfus Foundation
- Created
-
2018-05-16Created from EPrint's datestamp field
- Updated
-
2021-11-15Created from EPrint's last_modified field