Ectodermal Wnt Function as a Neural Crest Inducer
Abstract
Neural crest cells, which generate peripheral nervous system and facial skeleton, arise at the neural plate/ectodermal border via an inductive interaction between these tissues. Wnts and bone morphogenetic proteins (BMPs) play roles in neural crest induction in amphibians and zebrafish. Here, we show that, in avians, Wnt6 is localized in ectoderm and in vivo inhibition of Wnt signaling perturbs neural crest formation. Furthermore, Wnts induce neural crest from naı̈ve neural plates in vitro in a defined medium without added factors, whereas BMPs require additives. Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos.
Additional Information
© 2002 American Association for the Advancement of Science. 12 February 2002; accepted 28 May 2002; published online 13 June 2002. Supported by NIH grant number NS36585. We thank C. Baker, A. Groves, and A. Knecht for helpful comments on this work.Attached Files
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Supplemental Material - 1070824S2_large.jpg
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Additional details
- Eprint ID
- 51911
- DOI
- 10.1126/science.1070824
- Resolver ID
- CaltechAUTHORS:20141118-131539860
- NIH
- NS36585
- Created
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2014-11-18Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field