Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published April 2012 | Accepted Version
Journal Article Open

Flexible microelectrode arrays to interface epicardial electrical signals with intracardial calcium transients in zebrafish hearts


The zebrafish (Danio rerio) is an emerging genetic model for regenerative medicine. In humans, myocardial infarction results in the irreversible loss of cardiomyocytes. However, zebrafish hearts fully regenerate after a 20% ventricular resection, without either scarring or arrhythmias. To study this cardiac regeneration, we developed implantable flexible multi-microelectrode membrane arrays that measure the epicardial electrocardiogram signals of zebrafish in real-time. The microelectrode electrical signals allowed for a high level of both temporal and spatial resolution (~20 μm), and the signal to noise ratio of the epicardial ECG was comparable to that of surface electrode ECG (7.1 dB vs. 7.4 dB, respectively). Processing and analysis of the signals from the microelectrode array demonstrated distinct ECG signals: namely, atrial conduction (P waves), ventricular contraction (QRS), and ventricular repolarization (QT interval). The electrical signals were in synchrony with optically measured Calcium concentration gradients in terms of d[Ca^(2+)]/dt at both whole heart and tissue levels. These microelectrodes therefore provide a real-time analytical tool for monitoring conduction phenotypes of small vertebral animals with a high temporal and spatial resolution.

Additional Information

© 2011 Springer Science+Business Media, LLC. Published online: 29 November 2011. The authors would like to express gratitude for Dr. Fuhua Chen from UCLA School of Medicine for providing the Calcium voltage mapping. This project was supported by the American Heart Association Pre-Doctoral Fellowship (11PRE7370088) (FY), National Institutes of Health, National Heart Lung and Blood Institute (HL083015) (TKH), HL091302 (TKH), (HL104239) (NCC) and National Institute of Child Health & Human Development (HD069305) (NCC and TKH).

Attached Files

Accepted Version - nihms366044.pdf


Files (2.5 MB)
Name Size Download all
2.5 MB Preview Download

Additional details

August 22, 2023
October 17, 2023