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Published February 16, 2009 | Erratum + Published
Journal Article Open

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging


Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO_3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and broad flexibility in the choice of excitation wavelength. We obtained monodispersed BaTiO_3 nanoparticles in colloidal suspensions by coating the particle surface with amine groups. We characterized the SHG efficiency of 90-nm BaTiO_3 particles experimentally and theoretically. Moreover, we use the coherent SHG signal from BaTiO_3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H^2) microscope which records digital holograms at the second harmonic frequency. For the first time, high-resolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H^2 microscope.

Additional Information

© 2009 Optical Society of America. Received 5 Jan 2009; revised 6 Feb 2009; accepted 7 Feb 2009; published 11 Feb 2009. Erratum published in Optics Express, v. 18:4 (doi:10.1364/OE.18.003456). We are very grateful to Scott E. Fraser and Periklis Pantazis for fruitful discussions on the SHG efficiency characterization. We are also grateful to Stephen Quake, Stavros Stavrakis, and Jerrod Schwartz for helpful discussions on the colloidal stabilization. We thank Paul Bowen for providing the BaTiO3 nanoparticles, Robin Humphry-Baker for the assistance with the light scattering measurement, and Marc Chambon for the help of HeLa cells preparation. The author C.-L. H. would like to thank Taiwan Merit Scholarship (TMS-094-2-A-042) for financial support. The author Y. P. would like to thank the Optofluidic Center for financial support. This project is supported by National Center of Competence in Research (NCCR), Quantum Photonics. OCIS Codes: (160.4330) Materials : Nonlinear optical materials; (170.6900) Medical optics and biotechnology : Three-dimensional microscopy; (320.7110) Ultrafast optics : Ultrafast nonlinear optics; (090.1995) Holography : Digital holography; (160.4236) Materials : Nanomaterials


Erratum published in Optics Express, v. 18:4. Original Manuscript: January 28, 2010. Published: February 2, 2010. Chia-Lung Hsieh, Rachel Grange, Ye Pu, and Demetri Psaltis, "Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging: erratum," Opt. Express 18, 3456-3457 (2010) http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-4-3456

Attached Files

Published - Hsieh2009p98.pdf

Erratum - Hsieh2010p7200Opt_Express-erratum.pdf


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August 20, 2023
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