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Published February 22, 2021 | Supplemental Material + Submitted + Accepted Version + Published
Journal Article Open

Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules


Drosophila reproductive behaviors are directed by fruitless neurons. A reanalysis of genomic studies shows that genes encoding dpr and DIP immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. We find that each fru P1 and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes indicate that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of fru P1 neurons shows that many DIPs have high expression in a small set of neurons, whereas the dprs are often expressed in a larger set of neurons at intermediate levels, with a myriad of dpr/DIP expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of DIP-ε change the sex-specific morphologies of fru P1 ∩ DIP-α neurons.

Additional Information

© 2021 Brovero et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Received: 17 September 2020; Accepted: 22 February 2021; Published: 22 February 2021. The work presented was supported by NIH grants awarded to MNA: R01GM073039, R01GM116998, R03NS090184. This work was also supported by funds from the Biomedical Sciences Department, College of Medicine, Florida State University. We are grateful for the support. We appreciate that colleagues sent Drosophila stocks (Key Resources Table). Stocks were also obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537). Several antibodies used in this study were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. We thank Batory foods (Lithia Springs, GA) for the cornmeal used in the fly media. We appreciate experimental assistance from Catherina Artikis. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Author contributions: Savannah G Brovero, Julia C Fortier, Hongru Hu, Pamela C Lovejoy, Nicole R Newell, Formal analy- sis, Validation, Investigation, Writing - original draft, Writing - review and editing; Colleen M Palmat- eer, Ruei-Ying Tzeng, Formal analysis, Validation, Methodology, Writing - original draft, Writing - review and editing; Pei-Tseng Lee, Resources; Kai Zinn, Resources, Writing - review and editing; Michelle N Arbeitman, Conceptualization, Formal analysis, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review and editing. Data availability: All raw data are provided in the supplementary materials. The sequencing data have been deposited in GEO under accession number GSE162098.

Attached Files

Published - elife-63101-v2.pdf

Accepted Version - elife-63101-v1.pdf

Submitted - 2020.10.02.323477v1.full.pdf

Supplemental Material - elife-63101-supp-v1.zip

Supplemental Material - elife-63101-transrepform-v2.docx


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August 20, 2023
December 22, 2023