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Published February 1940 | Published
Journal Article Open

The formation of creatine from glycocyamine in the liver


The study of the precursors of creatine in animals has been beset by two difficulties principally. One has been the lack of really adequate biological material; the other, the lack of a specific, and at the same time sensitive analytical method. Experiments hitherto have consisted in attempts to change the urinary excretion of creatine and creatinine, or the creatine content of the tissues of intact animals or of isolated perfused organs. The normal, i.e. uncontrolled, fluctuations in tissue composition and urinary excretion are relatively large compared with the changes induced experimentally; it is often impossible to distinguish when experimental effects are observed, whether these have arisen from changes in the processes of excretion or synthesis; there may be variations in the water content of the tissues, thereby affecting their percentile composition; all of these have stood in the way of firm conclusions being drawn.

Additional Information

© 1940 American Society of Biological Chemists. Received for publication, November 15, 1939. The authors wish to thank Mr. Y. Tajima for the assistance he gave them throughout this work, and Dr. H.W. Davenport for advice and assistance in the construction of the shaker used here. They are indebted to Dr. R. Dubos, and wish to thank him for specimens of the bacteria used in these experiments and valuable information on the culture details.

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