Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

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Acknowledgement

This work was supported by the Institute for Basic Science (IBS-R026-D1, to J.-H.L, M.K., S.-W.C. and J.C.). This work was also supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government, the Ministry of Science and ICT (MSIT) (numbers 2021R1A2C3004262 and 2022M3A9B6082675) and Samsung Research Funding & Incubation Center of Samsung Electronics under project number SRFC-TC2003-03 to S.-W.C. This research was also supported by the Yonsei Signature Research Cluster Program of 2023-22-0012 and the Yonsei Fellow Program funded by Lee Youn Jae to S.-W.C. This work was also supported by the US National Institutes of Health (R01-EB018975) to M.G.S. E.C.-H. was supported by the James G. Boswell Fellowship in Molecular Engineering and MRI. M.G.S. is an Investigator of the Howard Hughes Medical Institute (HHMI).

This article is subject to HHMI's Open Access to Publications policy. HHMI Investigators have previously granted a non-exclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication.

Contributions

These authors contributed equally: Whee-Soo Kim, Sungjin Min.

W.-S.K. performed overall in vitro and in vivo experiments and analysed data. S.M designed and performed organoid-related experiments. S.K.K., Y.H.K. and S.A. supported hiPSC differentiation, MMUS imaging and hydrogel characterization, respectively. S.K. provided magnetic nanoparticles. E.C.-H. supported the measurements of ultrasound signals using hydrophones. H.D. and A.B.-Z. performed initial set-up and discussions on this research. D.M. provided gas vesicles. J.-H.L. helped with initial set-up and discussions on this research. S.H.B. and J.G.L. provided lung tissue for the organoid development. M.K. assisted with the design of research and experiment. W.-S.K and M.K. wrote the manuscript with input from all authors. S.-W.C., M.G.S. and J.C. conceived and supervised the project.

Data Availability

The data presented in this study are available in the Source data. Additional information and requests for resources and reagents that support the findings of this study are available from the corresponding author upon reasonable request. Source data are provided with this paper.

Code Availability

Ultrasound data acquisition and analysis code is available on the Shapiro laboratory GitHub at https://github.com/shapiro-lab.

Conflict of Interest

The authors declare no competing interests.