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Published October 13, 2015 | Published + Supplemental Material
Journal Article Open

Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells


Premigratory neural crest cells comprise a transient, embryonic population that arises within the CNS, but subsequently migrates away and differentiates into many derivatives. Previously, premigratory neural crest could not be maintained in a multipotent, adhesive state without spontaneous differentiation. Here, we report conditions that enable maintenance of neuroepithelial "crestospheres" that self-renew and retain multipotency for weeks. Moreover, under differentiation conditions, these cells can form multiple derivatives in vitro and in vivo after transplantation into chick embryos. Similarly, human embryonic stem cells directed to a neural crest fate can be maintained as crestospheres and subsequently differentiated into several derivatives. By devising conditions that maintain the premigratory state in vitro, these results demonstrate that neuroepithelial neural crest precursors are capable of long-term self-renewal. This approach will help uncover mechanisms underlying their developmental potential, differentiation and, together with the induced pluripotent stem cell techniques, the pathology of human neurocristopathies.

Additional Information

© 2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Received: September 8, 2014; Revised: August 27, 2015; Accepted: August 28, 2015; Published: October 1, 2015. We thank Drs. Chathurani Jayasena and Crystal Rogers and Erin Moran for technical assistance. This work was funded by DE024157 (to M.E.B.) and the Sigrid Juselius Foundation, Finnish Cultural Foundation, Jane and Aatos Erkko Foundation, Väre Foundation, and Ella and Georg Ehrnrooth Foundation (to L.K.).

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Published - 1-s2.0-S2213671115002519-main.pdf

Supplemental Material - mmc1.pdf


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August 20, 2023
August 20, 2023