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Published June 1, 1983 | public
Journal Article Open

Genetic Mapping in the Major Histocompatibility Complex by Restriction Enzyme Site Polymorphisms: Most Mouse Class I Genes Map to the Tla Complex


From a genomic library constructed from sperm DNA of the inbred BALB/c mouse, we previously isolated 54 cosmid clones that contain 36 class I genes and can be divided by restriction map analyses into 13 gene clusters. We have isolated single- and low-copy DNA probes from each of these clusters to visualize restriction enzyme site polymorphisms in the DNAs from various congeneic and recombinant congeneic mice. These polymorphisms permit us to map each of the 13 cosmid clusters to a precise location in the major histocompatibility complex of the mouse. Thirty-one of 36 class I genes map into the Tla complex of the major histocompatibility complex whereas the remaining 5 genes map to the H-2 complex. Thus, all 36 class I genes are located in the major histocompatibility complex. Analysis of the number of restriction enzyme fragments visualized by the single- and low-copy DNA probes suggests that the class I genes in different inbred strains of mice probably undergo gene duplications and deletions, presumably by homologous but unequal crossing-over.

Additional Information

Copyright © 1983 by the National Academy of Sciences Contributed by Leroy Hood, February 15, 1983 We thank Karyl Minard for excellent technical help, Mary J. Macchi and Dr. Jeff Frelinger for some of the recombinant congeneic mouse DNAs used in the Southern blot analyses, and Bernita Larsh for the preparation of the manuscript. We also thank Henry Sun, Drs. Steve Crews, Joan Kobori, Marie Malissen, Bernard Malissen, Roger Perlmutter, and Martha Zuniga for comments on the manuscript. A.W was supported by a fellowship from the California Foundation for Biochemical Research. M.S. was supported by a Senior Leivre Fellowship from the California Division of the American Cancer Society. The work was supported by grants from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.


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