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Published March 2020 | Accepted Version + Supplemental Material
Journal Article Open

Snapshot photoacoustic topography through an ergodic relay for high-throughput imaging of optical absorption


Current embodiments of photoacoustic imaging require either serial detection with a single-element ultrasonic transducer or parallel detection with an ultrasonic array, necessitating a trade-off between cost and throughput. Here, we present photoacoustic topography through an ergodic relay (PATER) for low-cost high-throughput snapshot wide-field imaging. Encoding spatial information with randomized temporal signatures through ergodicity, PATER requires only a single-element ultrasonic transducer to capture a wide-field image with a single laser shot. We applied PATER to demonstrate both functional imaging of haemodynamic responses and high-speed imaging of blood pulse wave propagation in mice in vivo. Leveraging the high frame rate of 2 kHz, PATER tracked and localized moving melanoma tumour cells in the mouse brain in vivo, which enabled flow velocity quantification and super-resolution imaging. Among the potential biomedical applications of PATER, wearable devices to monitor human vital signs in particular is envisaged.

Additional Information

© 2020 Springer Nature Limited. Received: 3 April 2018; Accepted: 4 December 2019; Published: 20 January 2020. We thank J. Ballard and C. Ma for close reading of the manuscript, Y. He and C. Yeh for technical support, and P. Hai for his image superposition codes. This work was sponsored by National Institutes of Health Grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), R01 EB016963, U01 NS090579 (NIH BRAIN Initiative) and U01 NS099717 (NIH BRAIN Initiative). Author Contributions: Y.L. and L.L. designed the study. Y.L., L.L. and K.M. built the imaging system. L.L. and Y.L. planned the experiments. Y.L., L.L., E.B. and J.Y. performed the experiments. J.S. and L.W. developed the data acquisition program. L.Z., Y.L. and L.L. developed the reconstruction algorithm. Y.L., L.L., L.Z., J.L., P.H. and J.Y. analysed the data. L.V.W. conceived the concept and supervised the project. All authors contributed to writing the manuscript. Data availability: The data that support the plots within this paper and other findings of this study are available from the corresponding author upon reasonable request and with permission from corporate collaborations. Code availability: The reconstruction algorithm and data processing methods are described in detail in the Methods. We have opted not to make the computer codes publicly available owing to corporate collaborations and pending patent applications. Competing interests: L.V.W. and K.M. have financial interests in Microphotoacoustics, Inc., CalPACT, LLC and Union Photoacoustic Technologies, Ltd, which did not support this work.

Attached Files

Accepted Version - nihms-1590103.pdf

Supplemental Material - 41566_2019_576_MOESM10_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM11_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM1_ESM.pdf

Supplemental Material - 41566_2019_576_MOESM2_ESM.pdf

Supplemental Material - 41566_2019_576_MOESM3_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM4_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM5_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM6_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM7_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM8_ESM.mp4

Supplemental Material - 41566_2019_576_MOESM9_ESM.mp4


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Additional details

August 19, 2023
October 18, 2023