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Published March 2015 | public
Journal Article

Luminescent ruthenium probe for DNA mismatches


Our lab. has focused on the design of metalloinsertors in an effort to prep. new chemotherapeutic agents targeted towards mismatch repair (MMR) - deficient cells. Metalloinsertors bind selectively to DNA mismatches and show preferential selectivity in MMR- deficient cells. We have also been interested in designing luminescent ruthenium complexes that could function as diagnostic probes for DNA mismatches. It is known that the complex [Ru(bpy)_2(dppz)]^(2+) while non- emissive in aq. soln., exhibits a restored luminescence upon binding to duplex DNA. In this work, a new sterically expansive ligand (BNIQ) was synthesized and coordinated to ruthenium to create a new luminescent complex [Ru(bpy)_2(BNIQ)]^(2+). The luminescent properties of this complex in the presence of well- matched and mismatched DNA duplexes were examd. using steady state and emission lifetime measurements. The complex exhibits an enhanced luminescence in the presence of mismatched DNA relative to well- matched DNA, suggesting that this complex may be utilized as a novel probe for DNA mismatches.

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© 2015 American Chemical Society.

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