Lyt-2 glycoprotein is synthesized as a single molecular species
- Creators
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Rothenberg, E.
- Triglia, D.
Abstract
We investigated the possibility that the Lyt-2 molecules made by uncloned mouse T lymphocytes would show variable primary structures like those of immunoglobulins. Newly synthesized Lyt-2/3 complexes were found to include only two major components, both discrete glycoproteins with apparent molecular weights of 31,000 (31 K) and 35,000 (35 K). When products of Lyt-2.1 and Lyt-2.2 thymocytes were compared by two-dimensional nonequilibrium pH gradient electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis, the isoelectric points of the 35 K molecules were different; thus, the 35 K component was likely to be encoded by the Lyt-2 locus itself. However, the 35 K molecules made by any one genotype were homogeneous in charge as well as in size. The homogeneity was obscured rapidly by post-translational modification. Most strikingly, within 30 min of initial synthesis, these processing events generated the conspicuous array of microheterogeneous products that form the "38 K" component of cell-surface Lyt-2/3.
Additional Information
© 1983 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Supported by grants CA 31667, AI 16769, AI 19752, and CA 14195 from the National Institutes of Health. We would like to thank Dr. Susan Swain and Dr. Richard Dutton for congenic mice and Dr. Leonard Herzenberg and Dr. Ulrich Hammerling for monoclonal antibodies. Endo H was the generous gift of Dr. Phillips Robbins.Files
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Additional details
- PMCID
- PMC2186898
- Eprint ID
- 74212
- Resolver ID
- CaltechAUTHORS:20170210-131601098
- CA31667
- NIH
- AI16769
- NIH
- AI19752
- NIH
- CA14195
- NIH
- Created
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2017-02-11Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field