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Published October 1, 2013 | Accepted Version + Supplemental Material
Journal Article Open

Fast Metabolic Response to Drug Intervention Through Analysis on a Miniaturized, Highly Integrated Molecular Imaging System


We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs. Methods: A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to ^(18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling. Results: The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis. Conclusion: The kinetic analysis enabled by the platform provided a rapid (∼1 h) drug screening assay.

Additional Information

© 2013 Society of Nuclear Medicine and Molecular Imaging, Inc. Received Dec. 20, 2012; revision accepted May 14, 2013. Published online Aug. 26, 2013. The costs of publication of this article were defrayed in part by the payment of page charges. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 USC section 1734. This work was funded by the National Cancer Institute (5U54 CA119347, to James R. Heath), the National Institute of Neurological Disorders and Stroke (NS 73831, to Paul Mischel), the Ben and Catherine Ivy Foundation, and the Department of Energy (DE-SC0001249). Kiwook Hwang acknowledges the Samsung Foundation for a fellowship. Some of the authors (Caius Radu, Arion F. Chatziioannou, Michael E. Phelps, and James R. Heath) have ownership in Sofie Biosciences, which has rights to the ^(18)F-FAC probe. No other potential conflict of interest relevant to this article was reported. We thank David Baltimore for access to the Nikon Diaphot 200 fluorescence microscope.

Attached Files

Accepted Version - nihms589077.pdf

Supplemental Material - 118497_Supplemental_Data.pdf


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