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Published March 2, 2010 | Published
Book Section - Chapter Open

In vivo, dual-modality imaging of mouse eyes: optical coherence tomography and photoacoustic microscopy within a single instrument


Optical-resolution photoacoustic microscopy (OR-PAM) provides superb optical absorption contrast for red blood cells (RBCs), which makes it ideal for in vivo microvasculature imaging. In comparison, optical coherence tomography (OCT), widely used for tissue microstructure imaging, provides high optical scattering contrast. The two contrast mechanisms are highly complementary. In this work, we combined OR-PAM and OCT into a single, dual-modality imaging instrument for in vivo mouse eye imaging. We demonstrated in vivo dual-modality imaging of the anterior segment of mouse eyes with laser pulse energy within the ANSI laser safety standard.

Additional Information

© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). The OCT/PAM dual-modality system and mouse ocular anterior segment imaging demonstration were contributed in equal measure by Bin Rao and Li Li. The research was supported by the National Institutes of Health grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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