Identification of a Second Collagen-Like Glycoprotein Produced by Bacillus anthracis and Demonstration of Associated Spore-Specific Sugars
Abstract
Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.
Additional Information
© 2005, American Society for Microbiology. Received 7 February 2005/ Accepted 28 March 2005. The work was supported by NIH grant 1R21 AI05943 to G. Stewart and A. Fox. M. Stump was partially supported by the University of South Carolina Centenary Fund and Savannah River National Laboratory (SCUREF, WSCRC SC0178) and NIH R01 B0055201 (M. Matthews). We thank Bob Price and Jeff Davis for electron microscopic characterization of spores.Files
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Additional details
- PMCID
- PMC1151769
- Eprint ID
- 1852
- Resolver ID
- CaltechAUTHORS:WALjbact05
- NIH
- 1R21 AI05943
- University of South Carolina
- Savannah River National Laboratory
- NIH
- R01 B0055201
- Created
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2006-02-20Created from EPrint's datestamp field
- Updated
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2023-06-01Created from EPrint's last_modified field