Section-illumination photoacoustic microscopy for dynamic 3D imaging of microcirculation in vivo

Creators: Song, Liang; Maslov, Konstantin; Wang, Lihong V.

Abstract

We developed section-illumination photoacoustic microscopy capable of dynamic in vivo imaging of microvessels as small as 30 μm in diameter. The section illumination improved the elevational resolution while an ultrasound array provided the in-plane axial and lateral resolutions. Using the imaging system, we monitored the wash-in dynamics of Evans Blue in the microcirculation of mouse ears at 249 Hz 2D and 0.5 Hz 3D image acquisition rates. Such observation allowed us to differentiate the arterioles from the venules. In the future, the technology may be used to study angiogenesis, diabetes-induced vascular complications, and pharmacokinetics.

Additional Information

© 2010 Optical Society of America. Received February 17, 2010; accepted March 18, 2010; posted March 31, 2010 (Doc. ID 124273); published April 30, 2010. This work was sponsored in part by NIH grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. The authors thank Chulhong Kim, Song Hu, Junjie Yao, and Changhui Li for beneficial discussions and thank Professor James Ballard for close reading of the mansucript.

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