Identification of an embryonic isoform of myelin basic protein that is expressed widely in the mouse embryo
We have identified a myelin basic protein (MBP) isoform in mouse embryos that includes an exon upstream of the usual transcription initiation site. This isoform, embryonic-neonatal MBP (E-MBP), is expressed at the protein level in the embryonic nervous system at a time when other MBP isoforms are not detected. In addition to the central and peripheral nervous systems of the embryo and neonate, the thymus, spleen, and testes also express E-MBP at the protein level. The expression of E-MBP in cell types distinct from the nervous system strongly suggests that this MBP isoform has a role apart from the formation of myelin.
Additional Information© 1993 National Academy of Sciences. Contributed by Leroy Hood, June 28, 1993. We thank Drs. Michael Harrington, Joan Kobori, Laura Lebow, Sally Orr, Lance Fors, and Ray Owen for their insightful discussions and suggestions regarding this research. We thank Dr. Suzanna Horvath for peptide synthesis. We thank Kristine Engelbrecht for excellent technical assistance. This work was supported, in part, by National Institutes of Health Grant 195 AI1643307. P.M.M. is an Advanced Postdoctoral Fellow of the National Multiple Sclerosis Society (FG 939-A-1). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Published - PNAS-1993-Mathisen-10125-9.pdf