On Limits of Performance of DNA Microarrays
- Creators
- Vikalo, H.
- Hassibi, B.
- Hassibi, A.
Abstract
DNA microarray technology relies on the hybridization process which is stochastic in nature. Probabilistic cross-hybridization of non-specific targets, as well as the shot-noise originating from specific targets binding, are among the many obstacles for achieving high accuracy in DNA microarray analysis. In this paper, we use statistical model of hybridization and cross-hybridization processes to derive a lower bound (viz., the Cramer-Rao bound) on the minimum mean-square error of the target concentrations estimation. A preliminary study of the Cramer-Rao bound for estimating the target concentrations suggests that, in some regimes, cross-hybridization may, in fact, be beneficial—a result with potential ramifications for probe design, which is currently focused on minimizing cross-hybridization.
Additional Information
© Copyright 2006 IEEE. Reprinted with permission. [Posted online: 2006-07-24] This work was supported in part by the National Science Foundation under grant no. CCR-0133818 and CCR-0326554, by the David and Lucille Packard Foundation, and by Caltech's Lee Center for Advanced Networking.Files
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Additional details
- Eprint ID
- 9073
- Resolver ID
- CaltechAUTHORS:VIKicassp06
- Created
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2007-10-25Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field