Intermediate conformations of CD4-bound HIV-1 Env heterotrimers

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Acknowledgement

We thank W. Mothes, W. Li and Z. Qin (Yale University) for sharing cryo-ET data; J. Vielmetter, A. Rorick, K. Storm and the Protein Expression Center in the Beckman Institute at Caltech for expression assistance; J. E. Robinson (Tulane University) for the JR-52 antibody and A. DeLaitsch for comments on the manuscript. Electron microscopy was performed in the Caltech Cryo-EM Center with assistance from S. Chen. This work was supported by the National Institutes of Health (NIH) U54 AI170856 (P.J.B.), National Institute of Allergy and Infectious Diseases (NIAID) grant HIVRAD P01 AI100148 (to P.J.B.) and the Bill and Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery (CAVD) grant INV-002143 (P.J.B.). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NIAID or NIH.

Contributions

These authors contributed equally: Kim-Marie A. Dam, Chengcheng Fan.

K.A.D., C.F. and P.J.B. designed the research. K.A.D. designed Env constructs, performed protein purification and conducted ELISAs. C.F. collected structural data. Z.Y. created Supplementary Video 1. K.A.D., C.F., Z.Y. and P.J.B. analysed results. K.A.D. and P.J.B. wrote the manuscript with input from co-authors.

Data Availability

The cryo-EM maps and atomic structures have been deposited in the Protein Data Bank (PDB) and/or Electron Microscopy Data Bank (EMDB) under accession codes 8FYI and EMD-29579 for class I CD4–BG505 HT1, EMD-40437 for class II CD4–BG505 HT1, EMD-40438 for class III BG505 HT1, 8FYJ and EMD-29580 for class I CD4–BG505 HT2, EMD-29581 for class II CD4–BG505 HT2, EMD-29582 for class III CD4–BG505 HT2, EMD-29601 for CD4–B41 HT2, EMD-29583 for CD4–17b–BG505 HT1 and EMD-29584 for CD4–17b–BG505 HT2. PDB entries (6UDJ, 5U1F, 7LOK, 7TFN, 5VN3 and 6CM3) used in this study were downloaded from the PDB. EMDB entry EMD-21411 used in this study was downloaded from the EMDB.

Conflict of Interest

The authors declare no competing interests.

Errata

In the version of the article initially published, reference 11 included a preprint title and DOI, and has now been amended to reflect the published article (Li, W. et al. HIV-1 Env trimers asymmetrically engage CD4 receptors in membranes. Nature 623, 1026–1033 (2023)) in the HTML and PDF versions of the article.