A sea urchin genome project: Sequence scan, virtual map, and additional resources
Results of a first-stage Sea Urchin Genome Project are summarized here. The species chosen was Strongylocentrotus purpuratus, a research model of major importance in developmental and molecular biology. A virtual map of the genome was constructed by sequencing the ends of 76,020 bacterial artificial chromosome (BAC) recombinants (average length, 125 kb). The BAC-end sequence tag connectors (STCs) occur an average of 10 kb apart, and, together with restriction digest patterns recorded for the same BAC clones, they provide immediate access to contigs of several hundred kilobases surrounding any gene of interest. The STCs survey >5% of the genome and provide the estimate that this genome contains approx 27,350 protein-coding genes. The frequency distribution and canonical sequences of all middle and highly repetitive sequence families in the genome were obtained from the STCs as well. The 500-kb Hox gene complex of this species is being sequenced in its entirety. In addition, arrayed cDNA libraries of >105 clones each were constructed from every major stage of embryogenesis, several individual cell types, and adult tissues and are available to the community. The accumulated STC data and an expanding expressed sequence tag database (at present including >12,000 sequences) have been reported to GenBank and are accessible on public web sites.
Additional InformationCopyright © 2000 by the National Academy of Sciences Contributed by Eric H. Davidson, June 7, 2000. Article published online before print: Proc. Natl. Acad. Sci. USA, 10.1073/pnas.160261897. This project was supported by the Stowers Institute for Medical Research, to which the whole field of sea urchin molecular/developmental biology is pleased to acknowledge its gratitude. J.P.R. was supported by a National Institutes of Health Individual National Research Service Award (GM-18478). The Caltech Beckman Institute provided invaluable support for the robotics arraying facility essential to this project. R.A.C. and G.M. contributed equally to this work. Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AZ135866-AZ211804).
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