Noninvasive, in vivo imaging of the mouse brain using photoacoustic microscopy
Noninvasive, high resolution imaging of mouse brain activity is poised to provide clinically translatable insights into human neurological disease progression. Toward noninvasive imaging of brain activity through the hemodynamic response, the dark-field photoacoustic microscopy (PAM) technique was enhanced to image the cortex vasculature of the mouse brainin vivo using endogenous hemoglobin contrast. Specifically, the PAM system was redesigned to efficiently collect photoacousticwaves originating from cortical vessels, providing high (70μm lateral and 54 μm axial) resolution images of the mouse brain vasculature with a contrast-to-noise ratio of 25 dB. These findings confirm the efficacy of PAM to noninvasively image vascular structures in the mouse brain and the potential to image mouse brain function by tracking the hemodynamic response.
Additional Information© 2009 American Institute of Physics. Received 25 April 2008; accepted 12 July 2008; published online 19 May 2009. This work was sponsored by NIH Grant Nos. R01 EB000712 and R01 NS46214.
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