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Published June 1, 1952 | public
Journal Article Open

Incorporation in vitro of labeled amino acids into proteins of rabbit reticuloytes


Continuing our work on the incorporation of labeled amino acids into proteins (1), we have begun a study of the incorporation in vitro of C14-labeled glycine, L-histidine, L-leucine, and L-lysine into the proteins of rabbit reticulocytes. In preliminary experiments the incorporation into the hemoglobin isolated from the reticulocytes was determined. But, after it was found that plasma contains factors accelerating amino acid incorporation, it was decided to proceed as rapidly as possible toward the identification of these factors; we have, therefore, measured incorporation into the total proteins of the reticulocytes, since isolation of the hemoglobin was time-consuming. The results obtained with hemoglobin and with the total proteins are essentially the same, indicating that the other proteins of the reticulocytes incorporate amino acids at approximately the same rate as hemoglobin.

Additional Information

Copyright © 2003 by the American Society for Biochemistry and Molecular Biology. (Received for publication, November 13, 1951) These studies were aided by a contract between the Office of Naval Research, United States Navy Department, and the California Institute of Technology, Division of Biology (NR-164-304). It was also supported (in part) by a research grant from the National Institutes of Health, United States Public Health Service, and by a grant from the American Cancer Society. The C14 used in this investigation was supplied by the Carbide and Carbon Chemicals Corporation, Oak Ridge, Tennessee, and obtained on allocation from the United States Atomic Energy Commission. A preliminary report of this work was given at the meeting of the Federation of American Societies for Experimental Biology, April 29 to May 3, 1951 (Federation Proc., 10, 18 (1951)). The folic acid was obtained from the Nutritional Biochemicals Corporation and the vitamin B12 from Chas. Pfizer and Company, Inc.


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