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Published March 4, 2014 | Published
Book Section - Chapter Open

Sub-diffraction-limited imaging by photobleaching imprinting microscopy


We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a two-fold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community.

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© 2014 Society of Photo-Optical Instrumentation Engineers.

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