Photoacoustic microscopy with 2-μm transverse resolution

Creators: Ku, Geng; Maslov, Konstantin; Li, Li; Wang, Lihong V.

Abstract

We present a new-generation optical-resolution confocal photoacoustic microscope, consisting of a 0.25–numerical aperture optical microscope objective and a 75-MHz center-frequency spherically focused ultrasonic transducer. Experiments verified that this microscope has a transverse resolution of 2μm, which is the highest to our knowledge among all photoacoustic imaging systems. In situ imaging of mouse ears shows the feasibility of resolving individual red blood cells in microvessels using the current system.

Additional Information

© 2010 SPIE. Paper 09322SSR received Jul. 31, 2009; revised manuscript received Dec. 16, 2009; accepted for publication Dec. 17, 2009; published online Mar. 25, 2010. We thank Younan Xia's group for providing gold nanocages. This work was supported in part by National Institutes of Health Grants No. R01 NS46214 (BRP), No. R01 EB000712, No. R01 EB008085, and No. U54 CA136398 (NTR). L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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