Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published May 18, 2001 | Supplemental Material
Journal Article Open

Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome


SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits—including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export—underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.

Additional Information

© 2001 American Association for the Advancement of Science. 12 February 2001; accepted 17 April 2001. Published Online May 3 2001. We thank C. Lois, J Roberts, T. Caspari, A. Carr, S. Forsburg, J. Thorner, T. Toda, F. Osaka, Y. Xiong, and P. Nurse for generously providing retroviral vectors, S. pombe strains, expression plasmids, and antibodies; M. Petroski for 32P-labeled ubiquitin; and R. Verma for experimental input, helpful discussions, and pointing out JAB1 homology to ubiquitin-specific proteases. We also thank P. Jackson and members of his laboratory for hosting this project in its early days. Supported by an Amgen fellowship (S.L.) and by the W. M. Keck Foundation and HHMI.

Attached Files

Supplemental Material - 10597801s1_large.jpg

Supplemental Material - 10597801s2_med.gif

Supplemental Material - 10597801s3_med.gif

Supplemental Material - 10597801s4_med.gif

Supplemental Material - 10597801s5_med.gif


Files (366.6 kB)
Name Size Download all
68.1 kB Preview Download
57.4 kB Preview Download
26.3 kB Preview Download
128.7 kB Preview Download
86.0 kB Preview Download

Additional details

August 19, 2023
October 18, 2023