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Published May 10, 1994 | metadata_only
Journal Article

Phosphorothioate oligonucleotide-directed triple helix formation


Phosphorothioate oligodeoxyribonucleotides were tested for their ability to recognize double-helical DNA in two distinct triple helix motifs. Purine-rich oligonucleotides containing a diastereomeric mixture of phosphorothioate or stereoregular (all RP) phosphorothioate linkages are shown to form triple-helical complexes with affinities similar to those of the corresponding natural phosphodiester oligonucleotides. In contrast, pyrimidine-rich phosphorothioate oligonucleotides containing a mixture of diastereomeric or stereoregular (all RP) linkages do not bind to double-helical DNA with measurable affinity. These observations have implications for triple helix structure and for biological applications.

Additional Information

© 1994 American Chemical Society. Received February 4, 1994; Revised Manuscript Received March 7, 1994. Abstract published in Advance ACS Abstracts, April 15, 1994. Supported by a predoctoral fellowship from the Howard Hughes Medical Institute (to J.G.H.) and by research grants from the NIH (AR40780 to B.J.W. and GM35724 to P.B.D.).

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August 20, 2023
August 20, 2023