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Published July 2, 1993 | public
Journal Article

G protein-coupled signal transduction pathways for interleukin-8


Interleukin-8 (IL-8) is one of the major mediators of the inflammatory response. The pathways by which IL-8 activates inositide-specific phospholipase C (PLC) were investigated by co-expression of different components of the guanosine triphosphate binding protein (G protein) pathway in COS-7 cells. Two distinct IL-8 receptors reconstituted ligand-dependent activation of endogenous PLC when transfected together with the G protein α subunits Gα 14, Gα 15, or Gα 16. However, reconstitution was not observed with cells that overexpressed Gα q or Gα 11. Furthermore, IL-8 receptors interacted with endogenous pertussis toxin-sensitive G proteins or with the recombinant G protein Gi to release free βγ subunits that could then specifically activate the β2 isoform of PLC. These findings suggest that IL-8 acts through signal-transducing pathways that are limited to specific heterotrimeric G proteins and effectors. These may provide suitable targets for the development of anti-inflammatory agents.

Additional Information

© 1993 American Association for the Advancement of Science. 11 January 1993; accepted 8 April 1993. We thank A. Katz for providing the Gβγ plasmids and T. Daly and J. Barry for purified IL-8 and GRO/MGSA. Supported by a U. S. Public Health Service grant (M. I. S.) and Repligen Corporation.

Additional details

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