Published June 2013
| Submitted
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Biomolecular resource utilization in elementary cell-free gene circuits
Abstract
We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical 'breadboard' validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular 'crosstalk' and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.
Additional Information
© 2013 AACC. We thank Z. Sun, C. Hayes, J. Kim, and E. Yeung for help with TX-TL experiments and useful discussions. We also thank J. B. Lucks for the untranslated RNA gene construct. This work is supported by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office, and the DARPA Living Foundries Program under Contract HR0011-12-C-0065. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.Attached Files
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Additional details
- Eprint ID
- 39352
- Resolver ID
- CaltechAUTHORS:20130715-075915746
- Army Research Office (ARO)
- W911NF-09-0001
- Defense Advanced Research Projects Agency (DARPA)
- HR0011-12-C-0065
- Created
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2013-08-06Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field