Published 1988
| public
Journal Article
H+-induced release of contents of phosphatidylcholine vesicles bearing surface-bound polyelectrolyte chains
- Creators
- Maeda, Mizuo
- Kumano, Atsushi
-
Tirrell, David A.
Abstract
A semisynthetic vesicular membrane was constructed by immobilization of a synthetic polyelectrolyte [poly(2-ethylacrylic acid)] on the surface of a phosphatidylcholine bilayer. Immobilization was accomplished via Michael addition of polymer-bound thiol functions to a reactive maleimide handle incorporated into the lipid membrane in the form of dimyristoyl-N-[ [4-(maleimidomethyl)cyclohexyl]carbonyl]phosphatidylethanolamine. Semisynthetic membranes prepared in this way are sensitive to hydrogen ion concentration and are subject to large variations in permeability with small changes in pH. Rapid and quantitative release of vesicle contents can be achieved by mild acidification within the physiological pH range.
Additional Information
© 1988 American Chemical Society. Received February 8, 1988. This work was supported by a Presidential Young Investigator Award of the National Science Foundation (to D.A.T.) and by matching contributions from Air Products and Chemicals Co., Exxon Research and Engineering Co., General Electric Co., and Xerox Corp. We are grateful for leaves of absence granted by the University of Tokyo (to M.M.) and by the Japan Synthetic Rubber Co. (to A.K.).Additional details
- Eprint ID
- 53536
- Resolver ID
- CaltechAUTHORS:MAEjacs1988
- NSF
- Air Products and Chemicals Inc.
- Exxon Research and Engineering Co.
- General Electric
- Xerox Corporation
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2015-01-11Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field